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KMID : 0613820070170121682
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Journal of Life Science
2007 Volume.17 No. 12 p.1682 ~ p.1688
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Isolation and Characteristics of Bacteria Showing Biocontrol and Biofertilizing Activities
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Jung Ho-Il
Kim Keun-Ki
Park Hyean-Cheal
Lee Sang-Mong
Kim Yong-Gyun
Kim Hong-Sung
Son Hong-Joo
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Abstract
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To develop multifunctional microbial inoculant, microorganisms with antagonistic activity and biofertilizing activity were screened. Pantoea agglomerans and Bacillus megaterium from our laboratory culture collection, and strain MF12 from soil near poultry farm in Miryang were selected. On the basis of morphological, physiological studies and 16S rDNA sequence analysis, isolate MF12 was identified as the Bacillus pumilis. Three strains were studied for insoluble phosphate solubilization, indole-3-acetic acid (IAA) and siderophore production, ammonification ability, hydrolytic enzyme production and antifungal activity against phytopathogenic fungi. P. agglomerans did not produce any visible clear zone on agar plate containing 0.5% Ca3(PO4)2 as a sole phosphorus source. However, this strain could solubilize insoluble phosphate in liquid medium. All strains produced IAA ranged from 3~639 μg/ml depending on culture time and had ammonification ability. Among three strains, only P. agglomerans produced siderophore. P. agglomerans produced pectinase and lipase, B. megaterium produced amylase, protease and lipase while B. pumilis produced protease and lipase. P. agglomerans showed antifungal activities against phytopathogenic fungi, Fusarium oxysporum and Colletotrichum gloeosporioides. B. pumilis showed antifungal activities against Botrytis cinerea, Sclerotinia sclerotiorum and Phythium ultimum.
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KEYWORD
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Biofertilizer, biological control, multifunctional microbial inoculant, phytopathogenic fungi
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